Part:BBa_K925006

My Precious

This is a synthetically designed protein that is able to bind a mixture gold, silver, aluminium and titanium. It was designed as a scavenger protein due to it's ability to bind different metals. Its design was based around hijacking myoglobin's structure and replacing the loops and turns with our own metal binding peptides. This gave us a truly synthetic part for the registry.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 296
  Illegal XhoI site found at 468
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 318
  
• 1000
  COMPATIBLE WITH RFC[1000]

Description

When designing this part we based it around an existing protein, myoglobin, which had short α-helices and β-sheets. This allowed us to hijack the loops and turns of the myoglobin protein and inset our own metal binding sites. Some changes to the protein sequence was required as we used gBlocks^TM which are a maximum of 500 base pairs in length.

Characterisation

No characterisation has been done.

Results

No results have been found.

Conclusion

We have designed and ligated this sequence to form a BioBrick^TM but no expression or characterisation has occurred